recruiting

Chromatin from command or 60 min TNF-α-treated 3T3 cells was initially precipitated with α-IκBα . Chromatin was then simply eluted and a second precipitation with α-HDAC1 or -5 antibodies was performed . The presence of hes1 promoter in the precipitates was basically dependant on PCR analysis. Northern blot displaying relative hes1 transcriptional activation in IκBα+/+…